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Objective To evaluate the effects of 15 kinds of drying methods including sun-drying, shade drying, infrared drying (50, 60, 70, 80 ℃), microwave drying (50, 60, 70, 80, 100 ℃), and hot-air drying (50, 60, 70, 80 ℃) on the quality of Leonurus japonicus through the content of multiple chemical components, and then optimize suitable drying methods for L. japonicus. Methods UPLC-QTRAP®/MS2 method was developed to determine the content of three alkaloids (stachydrine hydrochloride, leonurine hydrochloride, trigonelline), four phenolic acids (benzoic acid, p-hydroxybenzoic acid, vanillic acid, syringic acid), five phenylpropanoids (salidroside, acteoside, chlorogenic acid, caffeic acid, ferulic acid), 11 flavonoids (rutin, isoquercitrin, hyperoside, wogonin, kaempferol-3-O-rutinoside, genkwanin, apigenin, kaempferol, isorhamnetin, hesperetin, quercetin), and one iridoid glycoside (ajugol) in L. japonicus. The principal component analysis (PCA) and TOPSIS analysis were performed to evaluate the quality of the L. japonicus samples obtained by different drying methods. Results Different drying methods exerted significant effects on the content of 24 chemical ingredients in L. japonicus. The PCA analysis divided 15 drying methods into three types based on the content of 24 compounds. Moreover, the comprehensive evaluation of TOPSIS was carried out, and the top three drying methods were 70 ℃ hot-air drying, 60 ℃ hot-air drying, and 100 ℃ microwave drying, which largely retained the active ingredients of L. japonicus. Conclusion Combined with practice, we found that 70 ℃ hot-air drying was the optimized drying process of L. japonicus, which provides guarantee for the quality of L. japonicus and provides scientific basis for the production and processing of L. japonicus.
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Objective To study the chemical constituents of the leaves of Callicarpa nudiflora. Methods The chemical constituents were isolated and purified by column chromatography on silica gel, MPLC, and PHPLC. Their structures were elucidated on the basis of physicochemical properties and spectroscopic analysis. Results Five compounds were isolated from the leaves of C. nudiflora and elucidated as 6’-O-caffeoyl-ajugol (1), luteolin (2), 5,4’-dihydroxy-3,7,3’-trimethoxyflavone (3), luteolin-4’-O-(6’’-E-caffeoyl)- β-D-glucopyranoside (4), and 2α,3α,19α,23-tetrahydroxyurs-12-en-28-ursolic acid (5). Conclusion Compound 1 is a new compound named nudifloside A1.
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OBJECTIVE: To study the chemical constituents of Callicarpa nudiflora. METHODS: The chemical constituents were isolated and purified by column chromatography on silica gel, ODS, Sephadex LH-20 and MPLC. Their structures were elucidated by spectroscopic evidence and compared with those in literature. RESULTS: Nine compounds were isolated and identified as 6-O-caffeoyl ajugol(1), leucosceptoslde A(2), 6-O-caffeoly-β-glucose(3), nudifloside(4), luteolin-7-O-glucoside(5), quercetin 3'-O-β-D-glucoside(6), cistaneside C(7), acteoside(8), and syringalide A 3'-α-L-rhamnopyranoside (9). CONCLUSION: Compounds 1, 2, 6, 7, and 9 are isolated from this plant for the first time.
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OBJECTIVE: To investigate the chemical constituents from the whole plants of Lagopsis supina. METHODS: The compounds were isolated and purified by various column chromatography, and their structures were identified based on their physiochemical properties and spectroscopic data. RESULTS: Thirteen compounds were isolated from the n-hexane, dichloromethane, and water extracts of the whole plants of Lagopsis supina by using various chromatographic methods. Their structures were identified as phytol(1), daucosterol(2), 8-O-acetylharpagide(3), antirrinoside(4), ajugoside(5), ajugol(6), harpagide(7), 1-O-caffeoyl-β-D-glucopyranose(8), 1-O-coumaroyl-β-D-glucopyranose(9), 2-hydroxy-5-(2-hydroxyethyl)phenyl-1-O-β-D-glucopyranoside(10), methyl 2-O-β-D-glucopyranosylbenzoate(11), adenosine(12), and sucrose(13), respectively. CONCLUSION: Compounds 1 and 3-13 are isolated from the plants of Lagopsis genus for the first time.
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The chemical study of Spathodea campanulata (Bignoniaceae) roots peels afforded an iridoid glucoside(ajugol) and two phenolic derivatives (p-hydroxy-benzoic acid and methyl p-hydroxy-benzoate). Thecompounds were characterized upon spectral data interpretation. Bioactivities of the constituents wereevaluated against fungus Cladosporium herbarum.
O estudo químico das cascas das raízes de Spathodea campanulata (Bignoniaceae) conduziu ao isolamentode um irióide glicosilado (ajugol) e dois derivados fenólicos (ácido p-hidroxi-benzóico e p-hidroxi-benzoato demetila). Os compostos foram identificados com base na interpretação dos seus dados espectrais. A atividadebiológica destes constituintes foi avaliada contra o fungo Cladosporium herbarum
Subject(s)
Bignoniaceae , Phenolic CompoundsABSTRACT
To study the chemical constituents of Buddleja lindleyana Fort.. Methods The constituents were isolated and purified by various chromatographic methods and structurally identifed by physico-chemical properties and spectral analysis. Results 10 compounds were obtained as α-spinasterol (Ⅰ), stigmasterol (Ⅱ)， β-sitosterol (Ⅲ), ursolic acid (Ⅳ), oleanolic acid (Ⅴ),phenanthrene (Ⅵ), glycerol mono tetracosanoate (Ⅶ), nonacosane (Ⅷ), acaciin (Ⅸ) and 6-O-vanilloyl-ajugol (Ⅹ). Conclusion All these compounds were obtained from this plant for the first time.